TABLE 1.

Comparative performance levels of the LIPS assay, Western blot analysis, and the Plexus immunoassay for serologic diagnosis of HSV infectiona

Assays and infection group% (no. of samples detected/no. positive)
SensitivitySpecificity
Western blot vs Plexus for HSV-1 (gG1)
    Uninfected100 (14/14)
    HSV-1100 (13/13)
    HSV-2100 (14/14)
    HSV-1/275 (9/12)
LIPS vs Western blot for HSV-1 (gG1)
    Uninfected100 (14/14)
    HSV-1100 (13/13)
    HSV-293 (13/14)
    HSV-1/283 (10/12)
LIPS vs Plexus for HSV-1 (gG1)
    Uninfected100 (14/14)
    HSV-1100 (13/13)
    HSV-293 (13/14)
    HSV-1/292 (11/12)
Western blot vs Plexus for HSV-2 (gG2)
    Uninfected93 (13/14)
    HSV-1100 (13/13)
    HSV-2100 (14/14)
    HSV-1/2100 (12/12)
LIPS vs Western blot for HSV-2 (gG2)
    Uninfected100 (14/14)
    HSV-1100 (13/13)
    HSV-2100 (14/14)
    HSV-1/2100 (12/12)
LIPS vs Plexus for HSV-2 (gG2)
    Uninfected93 (13/14)
    HSV-1100 (13/13)
    HSV-2100 (14/14)
    HSV-1/2100 (12/12)
  • a For LIPS assays, the cutoff limit for calculating sensitivity and specificity for each antigen was derived from the mean for the 14 uninfected samples plus 5 SD.