TABLE 2.

Temperature stability of flagellin-F1-Va

Assay temp (°C)DayTNF-α inductionbAdjuvant activityc
41581.8205/86
2133.2112/95
2970.9193/79
25291.8149/110
844115/93
1682167/77
37292.5107/93
846.2d71/86
16811d70/15d
  • a Aliquots of flagellin-F1-V were stored at 4°C, 25°C, or 37° for the indicated periods and were then analyzed for residual biologic activities.

  • b Samples at each time point were titrated in the in vitro RAW 264.7 cell assay for determination of the level of TNF-α production. The concentrations (10−11 M) required for 50% of the maximal response are shown.

  • c Samples were used at each time point to immunize BALB/c mice, as described in Materials and Methods. The levels of anti-F1 and -V in plasma were determined and compared to those in a day 0 control. The number before the slash is the F1 titer as a percentage of that for the day 0 control, and the number after the slash is the V titer as a percentage of that for the day 0 control.

  • d P < 0.001 by Mann-Whitney rank-sum test.