TABLE 3.

Flagellin-F1-V induces an antigen-specific adaptive immune response in nonhuman primatesa

SpeciesAnimal no.Preimmunization IgG titerPostimmunization IgG titer
Anti-F1Anti-VAnti-F1Anti-V
Adult African green monkeysb1<1,000<1,0004.9 × 1056.5 × 106
29.2 × 1037.2 × 1034.2 × 1054.8 × 106
3<1,000<1,0001.5 × 1058.4 × 105
45.2 × 1036.0 × 1036.0 × 1059.2 × 105
5<1,0004.1 × 1045.7 × 1052.0 × 106
67.6 × 1032.3 × 1035.7 × 1052.8 × 106
7<1,000<1,0002.8 × 1051.4 × 106
Young African green monkeysc13.3 × 103<1,0007.0 × 1041.5 × 105
2<1,000<1,0003.6 × 1058.6 × 105
3<1,0004.6 × 1049.1 × 1048.7 × 105
4<1,000<1,0003.1 × 1058.5 × 105
54.9 × 103<1,0008.6 × 1045.6 × 105
6<1,000<1,0001.0 × 1056.6 × 105
7<1,000<1,0002.1 × 1056.8 × 105
Cynomolgus monkeysd1<1,000<1,0001.6 × 1061.0 × 107
2<1,0006.2 × 1031.7 × 1069.4 × 106
3<1,000<1,0009.1 × 1058.2 × 106
49.5 × 103<1,0009.4 × 1046.0 × 105
5<1,000<1,0007.5 × 1056.1 × 106
  • a All of the monkeys were immunized i.m. twice with flagellin-F1-V and were then evaluated 2 weeks after the boost for the titers of anti-F1 and -V IgG antibodies in plasma.

  • b Adult monkeys immunized with 200 μg flagellin-F1-V.

  • c Monkeys ages 4 to 6 months immunized with 25 μg flagellin-F1-V.

  • d Immunized with 125 μg flagellin-F1-V.