TABLE 1.

Gene-specific primers for amplifying BbMSA-2c, BbRAP-1/CT, BbTRAP-T, BbSBP-1, and BbSBP-4

GeneOligonucleotide primera
BbMSA-2c5′-CGGAATTCATGGTGTCTTTAACATAATAACC-3′
5′-TAGCGGCCGCGAATGCAGAGAGAACGAAGTAGCAG-3′
BbRAP-1/CT5′-ACGGATCCGAGTTTTTCAGGGAA-3′
5′-ACCTCGAGAAACTCATGTATGAT-3′
BbTRAP-T5′-AGAATTCGAACCAAGCCGTGCTACACCG-3′
5′-ACTCGAGCTATTGTTTTTCGCCCTCGTAG-3′
BbSBP-15′-GCGAATTCAACGAAGCTGAGGTATCTCAG-3′
5′-GGCTCGAGTTAGTCTAGCATCTGTATTTT-3′
BbSBP-45′-AGGAATTCGAGGAGGAGGAAACTGATGAG-3′
5′-GCCTCGAGTTATTCCTCAATGTCGGCTGT-3′
  • a Each oligonucleotide primer includes restriction enzyme sites at the 5′ end (underlined), which are EcoRI and NotI for BbMSA-2c, BamHI and XhoI for BbRAP-1/CT, and EcoRI and XhoI for BbTRAP-T, BbSBP-1, and BbSBP-4.