TABLE 2.

ELISA titers of serum samples against recombinant proteins produced in three different heterologous expression systems

ImmunogenRabbitELISA titer against specified antigena
MSP-119 baculovirusMSP-119E. coliMSP-119PichiaAMA-1 PichiaAMA-1/MSP-119 fusion (Pichia)
IndividualMean (SD)IndividualMean (SD)IndividualMean (SD)IndividualMean (SD)IndividualMean (SD)
AMA-1 (Pichia)100 (0)00 (0)021,731* (37,639*)6,163,1615,211,353 (2,214,190)11,174,1547,528,464 (3,264,936)
80008,036,2039,562,225
150086,924*4,663,4076,819,924
200001,982,6392,557,553
AMA-1 and41,881,8861,747,361 (1,249,585)1,727,9021,905,354 (1,504,295)1,880,3431,896,859 (1,283,201)6,495,1464,906,408 (1,882,904)14,954,5709,674,828 (5,196,097)
    MSP-119 mut123,942,0914,704,9044,056,6897,529,25616,578,072
    M(Pichia)161,830,8011,891,1722,328,6464,514,9918,006,075
19706,647750,935790,7943,657,0635,256,569
26375,380451,857427,8222,335,5823,578,853
AMA-1/MSP-11957,416,3595,515,669 (1,761,481)7,634,7905,488,458 (1,879,153)7,333,8506,002,001 (1,968,330)7,490,8324,681,638 (2,285,393)20,378,26112,173,497 (5,034,747)
    fusion (Pichia)95,868,0075,021,6206,752,7503,795,04610,721,569
186,440,6896,011,8296,709,3972,596,6169,097,366
242,213,2212,136,3402,092,8712,205,8025,802,182
255,640,0676,637,7137,121,1347,319,89514,868,104
MSP-119614,926,68922,000,134 (14,134,561)12,521,50316,305,333 (9,595,486)12,213,57618,709,234 (12,338,749)00 (0)3,854,3694,911,977 (2,654,392)
    baculovirus738,288,77124,041,24029,029,43506,147,857
179,306,9376,874,4388,427,23001,699,378
237,670,3747,165,0416,350,23803,457,721
2739,807,89630,924,44437,525,69209,400,561
MSP-119 mut24,475,4433,129,648 (1,243,195)4,663,4693,649,773 (1,612,441)5,411,9534,183,259 (1,725,772)354,278*78,493* (138,235*)18,331,3615,811,042 (6,304,573)
    (Pichia)102,204,9771,984,8042,693,32502,129,327
133,113,2664,266,2224,388,54226,439*3,620,664
221,349,4131,567,2431,853,46801,612,972
284,505,1415,767,1286,569,00711,747*3,360,886
MSP-119 WT31,637,0032,041,431 (1,434,600)1,782,1171,774,597 (739,213)1,592,6361,744,707 (826,550)00 (0)706,355733,919 (243,088)
    (Pichia)11922,4461,120,2001,146,2320487,766
141,121,4371,218,1991,102,6840608,523
214,484,8372,977,8723,137,27501,133,032
  • a *, possible reactivity with Pichia contamination (possible reactivity with baculovirus contamination could not be tested).