TABLE 3.

ELISA of purified proteins collected showing a strong humoral response in animals immunized with the immunoreactive 49-kDa proteina

Well contentsaELISA result (optical density at 450 nm) for well containing serum at a dilution of:
None (pure serum)1:501:1001:2001:4001:8001:1,6001:3,2001:6,4001:12,8001:25,600
A0.000.000.000.000.000.000.000.000.000.000.00
B0.8830.8770.8640.8550.8520.8440.8400.8240.8070.8010.745
C0.5540.5410.5370.5360.5330.5310.5190.5170.5120.5110.475
D0.5220.5100.5070.5010.4980.4810.4790.4750.4740.4720.464
  • a A, blank (coated with coating buffer without any peptide); B, coating buffer containing 2 μg of immunoreactive protein P49A; C, 2 μg of the second purified protein (P49B); D, 2 μg of crude OMP (positive control).