TABLE 3.

Serotyping results for samples from patients with infections due to nonarchetypal strains

Case no.StrainPathologyGeographical originaOD index for:bGRA6 serotypecGRA6 C-terminal polymorphismdGenotype
GRA6 IIGRA6 I/III
24OcularCameroon0.0670.029NDI/III
25AsymptomaticFrance0.0070.002NDAtypical
26GUY-2004-TERCongenitalSuriname0.3720.471MixedGSVYAtypical
27GUY-2004-TERCongenitalSuriname0.3710.588MixedGSVYAtypical
28GUY-2004-TERMultivisceralSuriname0.0190.333I/IIIGSVYAtypical
29GUY-2004-TERMultivisceralSuriname0.484−0.022IIGSVYAtypical
30GUY-2004-TERMultivisceralSuriname0.052−0.009NDGSVYAtypical
31GUY-2004-TERMultivisceralSuriname0.0340.045NDGSVYAtypical
32GUY-2004-TERMultivisceralSuriname0.1390.586MixedGSVYAtypical
33GUY-2004-TERMultivisceralSuriname−0.0730.144I/IIIGSVYAtypical
34GUY-2004-TERMultivisceralSuriname0.1480.145MixedGSVYAtypical
35GUY-2004-TERAsymptomaticSuriname0.0190.009NDGSVYAtypical
36GUY-2004-TERAsymptomaticSuriname0.9432.346MixedGSVYAtypical
37GUY-2004-TERAsymptomaticSuriname0.0420.024NDGSVYAtypical
38GUY-2003-BASMultivisceralFrench Guiana0.1210.030IIGSVYAtypical
39GUY-2004-AKOMultivisceralFrench Guiana0.0540.838I/IIIEHVYAtypical
40GUY-2002-KOEMultivisceralFrench Guiana0.0220.476I/IIIERVYAtypical
41GUY-2002-MATMultivisceralFrench Guiana0.0761.241I/IIIERVYAtypical
  • a Cases 26 to 37 are from an outbreak of toxoplasmosis associated with the same strain (GUY-2004-TER).

  • b OD indices were calculated by subtracting the OD of the peptide control from the OD of each peptide. Cutoff values for African samples were 0.111 for GRA6 II and 0.079 for GRA6 I/III, for European samples were 0.088 for GRA6 II and 0.076 for GRA6 I/III, and for Latin American samples were 0.089 for GRA6 II and 0.067 for GRA6 I/III.

  • c ND, not determined because the serum sample had an OD index that was below the cutoff value; Mixed, serum sample reacted with both peptides.

  • d The amino acid polymorphisms from the C-terminal region of GRA6 are at positions 223, 224, 227, and 230.