TABLE 1.

Gene expression and cell viability following transfection of immature monkey DC

MethodDNAaRNAb
GFP expressioncViabilitydGFP expressionViability
TransFaste057 (3)7 (3)52 (4)
TransMessengere2 (1)49 (1)14 (8)55 (4)
Electroporationf4 (7)40 (2)9 (7)46 (3)
Nucleofectiong10 (4)24 (5)91 (3)68 (6)
  • a EGFP-N1 DNA.

  • b In vitro-transcribed gfp mRNA.

  • c Percentage of cells expressing GFP at 24 h posttransfection. Mean (SEM) of two to five experiments.

  • d Percentage of viable cells at 24 h posttransfection. Mean (SEM) of two to five experiments.

  • e A total of 4 μg DNA or mRNA was used with both lipofection methods.

  • f Cells were electroporated with 10 μg DNA or mRNA using 250 V/300 μF or 300 V/150 μF settings, respectively.

  • g Cells were nucleofected with 10 μg DNA or mRNA using the U-02 program.