TABLE 1.

IFN-γ and IL-10 production in PBMC cultures from pulmonary TB patients and healthy subjects

Subject(s)Cytokine production induced by indicated antigenIFN-γ/IL-10 production ratiobTB form
IFN-γ (pg/ml)aIL-10 (pg/ml)a
M. tuberculosisM. vaccaeCWSM. tuberculosisM. vaccaeCWSM. tuberculosisM. vaccaeCWS
TB patients
    A162,250.426,786.640,313.72,407.01,008.92,218.367.426.618.2Cavitary
    B32,222.22,791.53,768.4424.0148.4352.676.018.810.7Noncavitary
    C22,423.76,742.57,394.9379.3228.7675.959.129.510.9Noncavitary
    D3,143.0258.2922.0210.068.0125.315.03.87.4Noncavitary
    E1,001.0270.9229.9256.463.673.43.94.33.1Cavitary
    F2,758.847.874.72,272.41,153.01,859.91.20.00.0Noncavitary
    G1,423.194.5185.8740.4223.3543.41.90.40.3Cavitary
    H526.22.826.6187.7105.9138.92.80.00.2Cavitary
    I325.913.87.9861.4235.2876.10.40.10.0Cavitary
    J155.80.90.045.310.037.63.40.10.0Cavitary
    K27.80.71.0131.566.275.10.20.00.0Cavitary
    L25.90.010.685.837.5119.40.30.00.1Cavitary
    Medianc1,212.0&,¶71.1130.3*317.9&127.1245.8*
Healthy subjects (median)61.520.610.7101.357.879.7
  • a Minimum detectable concentrations were 2 pg/ml for IFN-γ and 0.5 pg/ml for IL-10.

  • b Ratios are the average IFN-γ values divided by the IL-10 value for each individual (5).

  • c Median levels of CWS-induced cytokines were significantly increased compared to those produced by M. vaccae (*, P < 0.05). Median levels of M. tuberculosis-induced cytokines were higher than those produced by M. vaccae (&, P < 0.001) or CWS (¶, P < 0.001), measured using the two-tailed Wilcoxon signed rank test.