TABLE 1.

PA-mediated toxicity is receptor dependent

Cell typeMean 7-AAD-positive cells ± SEMa
UntreatedWith PAWith PA+EFWith PA+LF
CHO-R1.12 ± 11 ± 12 ± 12 ± 1
CHO-K12 ± 32 ± 22 ± 22 ± 2
CHO-CMG22 ± 13 ± 17 ± 33 ± 2
CHO-TEM83 ± 224 ± 7*24 ± 12*15 ± 4*
  • a Cells were left untreated or incubated overnight with anthrax toxin components. Viability was analyzed by flow cytometry measuring 7-AAD permeability. Values represent the percentage of total cells exceeding two standard deviations of the mean of control cells. *, Statistically significant compared to untreated control cells (P < 0.01).