TABLE 1.

Specific activities of IgA, IgG, and IgM autoantibodiesa

AntigenSp actb of pooled CVL fluid samples from:
HIV-seronegative women (n = 22)cHIV-seropositive women (n = 24)
AIgAAIgGAIgMAIgAdAIgGeAIgMd
Actin0.070.703.403.081.1715.42
DNA0.801.052.966.500.8115.72
Myosin0.750.824.005.511.0515.36
Thyroglobulin0.550.944.163.340.459.96
Transferrin0.460.363.802.671.1711.10
  • a Autoantibody reactivities were detected by an indirect ELISA and were revealed with biotin-conjugated mouse Ig against human Fc according to their isotypes (IgA, IgG, and IgM), as described in Materials and Methods.

  • b Specific activities are in arbitrary units.

  • c The specific activities of antibodies of the IgA (AIgA), IgG (AIgG), or IgM (AIgM) isotype in CVL fluid to a given antigen were evaluated as the first dilution (1/d) that gave an OD between 0.5 and 1.5 and per μg of total IgA, IgG, or IgM, respectively, in the CVL fluid according to the formula A = (OD × d)/[Ig] (in μg/ml), as described previously (3, 40).

  • d The specific activities of the IgA and IgM NAbs in CVL fluid were higher in HIV-seropositive women than in HIV-seronegative women (P < 0.02).

  • e The specific activities of IgG NAbs in CVL fluid were similar in HIV-seronegative and HIV-seropositive women.