TABLE 3.

Pathological and microbiological findings following challenge of calves with M. bovis

Vaccine groupProportiona of animals with:Mean lesion scores ± SEM in:Mean no. of M. bovis culture-positive LNs/animal ± SEM
Lung lesionsLNb lesionsLungscLNsd
Nonvaccinated7/1010/103.1 ± 0.77.8 ± 1.12.5 ± 0.3
BCG alone5/104/10e1.1 ± 0.4e, f2.2 ± 1.1e1.6 ± 0.4
BCG-CFP-DDA-PIM23/102/10e0.9 ± 0.5e, f0.9 ± 0.6e, f1.7 ± 0.4
BCG-CFP-DDA-MPL3/105/10e0.6 ± 0.4e, f1.3 ± 0.6e1.7 ± 0.3
BCG-CFP-DDA-Pam3CSK41/10e, f2/10e0.3 ± 0.3e, f0.2 ± 0.1e, f1.3 ± 0.4e, f
BCG-DDA-MPL8/107/102.5 ± 0.53.8 ± 1.2e2.5 ± 0.3
  • a The data represent the number of animals with lung or lymph node lesions/total number of animals in the group.

  • b LN, lymph node.

  • c Lung lesion scores: 0, no lesions; 1, 1 to 9 lesions; 2, 10 to 29 lesions; 3, 30 to 99 lesions; 4, 100 to 199 lesions; 5, ≥200 lesions.

  • d Total lymph node lesion score per animal for individual nodes: 0, no lesions; 1, 1 to 19 small lesions (diameter, 1 to 4 mm); 2, ≥20 small lesions; 3, medium-size lesions (diameter, 5 to 9 mm); 4, large lesions (diameter, ≥10 mm). Scores for the four pulmonary lymph nodes for each animal were pooled.

  • e The results are significantly different from those for the nonvaccinated group (P < 0.05).

  • f The results are significantly different from those for the BCG-DDA-MPL group (P < 0.05).