TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype and/or characteristicsSource or reference
Strains
    S. enterica serovar Typhimurium χ8914ΔpabA1516 ΔpabB232 ΔasdA16; vaccine vectorLaboratory collection
    E. coli χ6097F araD139 Δ(proAB-lac) λ-Φ80dlacZΔM15 rpsL ΔasdA4 Δ(zhf-2::Tn10) thi-1; contains pYA232 (Tcr, lacIq); used for cloning and propagation of plasmid33
    C. perfringens
        CP995Type A, NE isolateG. Siragusa, USDA, Athens, GA
        JGS4143Type A, NE isolate, virulent strain5
Plasmids
    pYA3493Ptrc, asd, pBRori, bla SS; parent plasmid vector22
    pYA3620Ptrc, asd, pBRori, bla SS, bla CT; parent plasmid vector pYA3493 with bla C-terminal fusions22
    pYA4101Plpp, asd, pBRori, ompA SS; parent plasmid vector with OmpA signal peptide fusion
    pYA3977375-bp DNA encoding PlcC in pYA3493 PlcC expression plasmidThis study
    pYA4110375-bp DNA encoding PlcC in pYA4101 PlcC expression plasmidThis study
    pYA4149375-bp DNA encoding PlcC in pYA3620 PlcC expression plasmidThis study
    pYA3910375-bp DNA encoding PlcC in pBAD/HisB vector; used for His-tagged PlcC (rPlcC) expressionThis study