TABLE 2.

IFN-γ ELISPOT assay results at 8 weeks after initial vaccination

GroupaMean change in no. of IFN-γ SFCs/106 PBMCsb
Nonvaccinated35 ± 13
BCG131 ± 37
BCG-CFP-DDA-PIM2205 ± 51c
BCG-CFP-DDA-MPL212 ± 39c
BCG-CFP-DDA-Pam3CSK4213 ± 94c
BCG-DDA-MPL222 ± 48c
  • a Four animals per group were randomly selected for the IFN-γ ELISPOT assay.

  • b The change in the numbers of SFCs represents the number of SFCs for the bovine PPD-stimulated PBMCs minus the number of SFCs for the medium control PBMCs/106 cells. The mean number of SFCs for the medium control PBMCs/106 cells was 81 ± 19.

  • c The results are significantly different from the results for the nonvaccinated group (P < 0.05).