TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
    E. coli BL21(DE3)F′ ompT gal [dcm] [lon] hsdSB(rB mB) DE329
    Brucella abortus
        2308Bovine isolateB. Deyoe
        ADH4ΔvirB1-12::Km8
    Brucella melitensis
        16MWild type13
        BMΔasp24Δasp2413
        BMΔvirB2ΔvirB2 (nonpolar)13
        BMΔcydBAΔcydBA12
Plasmids
    pGEX-4T-2Apr, N-terminal GST tag, lacIqPharmacia
    pAV5.1VirB5 cloned into pGEX-4T-18
    pMO4VirB1 cloned into pGEX-4T-28
    pIVEX2.4bNdeIApr, T7 promoter, N-terminal six-His tagRoche
    pDS1VirB12 cloned into pIVEX2.4bNdeI31
    pT7-7Strep::VirB11VirB11 cloned into pT7-7Strep11