TABLE 1.

Reactivities of MAbs against Aspergillus by IFA, ELISA, and IHC

MAbIsotypeReactivity by IFAaA. fumigatus ELISA result (OD450)aReactivity by IHCa,b
A. fumigatusA. flavusA. nigerA. terreusA. nidulansABC
ConidiaHyphaeConidiaHyphaeConidiaHyphaeConidiaHyphaeConidiaHyphaeEAsMAs
MA7IgG3+++++++++++++++++++++++2.0382.304++++++++
EA4IgM++++++++++++++++1.5762.058+++++++
EA5IgM+++++++++++++++1.7772.284+++++++
EA8IgM++++++++++++++++++++++++1.9452.333++++++
MA4IgM+++++++++++++++++++++++2.2332.55+++++++
EA6IgM++++++++++++++++++++++1.9382.460++++++
EA2IgG1+++2.2612.861++++++
EA3IgG1++++2.3502.950+++
MA1IgG1+++1.6202.358++
EA1IgG1++++1.5382.407+++++
Con1IgM++++++++++++++++++++++1.7312.548+
Con2IgG3+++++++++++++++++++++1.6762.558+++
EA7IgM+++++++++++++++++++++++++2.3432.302++
MA2IgG1+++++++++++++++++++++++++++2.4321.957++++
MA3IgG1+++++++++++++++++++1.8932.668++++++
MA5IgG1+++++1.8412.513+++
MA6IgG1++++++++++++++++++++++++++2.3442.344+++
  • a The reactivity of each MAb was determined by IFA to the mycelia and conidia of Aspergillus species, by indirect ELISA to the MAs and EAs of A. fumigatus, and by immunohistochemical assay (IHC) to formalin-fixed paraffin section of tissues. −, no reactivity; +, weak reactivity; ++, intermediate reactivity; +++, strong reactivity; ++++, very strong reactivity.

  • b A, kidney tissue from an experimental rabbit model of IA; B, lung tissue from a patient with IA; C, lung tissue from a patient with aspergilloma.