TABLE 1.

Performance of the p24 Ag assay on all DBS after short- and long-term storage

AssayaNo. of children% Sensitivitye% Specificitye% PrevalenceLR
TotalWith false-negative resultsPositiveNegative
First
    Grade 1 paper
        Without desiccant124b,c296.5 (87.4, 99.7)100 (93.7, 100)44.40.0351
        With desiccant121d296.6 (87.6, 99.7)100 (93.3, 100)46.30.0345
    903 card
        Without desiccant125b296.6 (87.6, 99.7)100 (93.7, 100)44.80.0345
        With desiccant121d198.3 (89.3, 99.9)100 (93.3, 100)46.30.0175
Second
    Grade 1 paper
        Without desiccant124b,c394.8 (85.3, 98.8)100 (93.7, 100)44.40.0517
        With desiccant121d788.9 (78.8, 94.8)100 (93.3, 100)46.30.1111
    903 card0.082
        Without desiccant125b591.8 (81.8, 96.8)100 (93.7, 100)44.8
        With desiccant121d198.3 (89.8, 99.9)100 (93.3, 100)46.30.0175
  • a The first assay was performed within 6 weeks of sample collection, and the second assay was performed 6 weeks after the first.

  • b DBS from the first 125 children enrolled.

  • c No DBS on grade 1 paper from one child.

  • d DBS from the last 121 children enrolled.

  • e Values in parentheses are 95% confidence intervals.