TABLE 1.

Sensitivities of different capture ELISAs tested

Capture MAb (isotype)Detection MAbIsotypeSensitivitya
6D3 (IgG1)7H3IgAND
10F1IgA50 pg/ml
17C12IgG2aND
20B1 (IgG1)7H3IgAND
10F1IgA4 ng/ml
17C12IgG2a16.9 pg/ml
7H3 (IgA)6D3IgG1ND
20B1IgG1ND
17C12IgG2aND
10F1 (IgA)6D3IgG150 pg/ml
20B1IgG150 pg/ml
17C12IgG2a4 ng/ml
17C12 (IgG2a)6D3IgAND
20B1IgG1ND
7H3IgA40 ng/ml
10F1IgAND
  • a Sensitivity is defined by the lowest concentration of SEB from which the starting concentration (1 μg/ml) could be calculated by using the linear-fit standard curve generated in each ELISA. Antibody combinations without a sensitivity value listed did not successfully bind SEB in this assay; hence, calculations were not done (ND).