TABLE 2.

Results of ELISAs with MAbs 10F1 and 20B1 and MAbs 10F1 and 17C12

MAbs and quantity of SEB added (ng/ml)Quantity of SEB (ng/ml) determined in the following diluents:
1% BSA50% human serum50% mouse serum15 mg/ml mouse feces
MAbs 10F1 and 20B1
    37.0437.24 ± 0.01526.26 ± 0.00834.56 ± 0.003440.1 ± 0.005
    12.3513.71 ± 0.00115.60 ± 0.00316.59 ± 0.000324.89 ± 0.002
    4.123.94 ± 0.00024.61 ± 0.0015.28 ± 0.00038.89 ± 0.0007
    1.371.361.531.852.64 ± 0.0003
    0.460.500.560.751.17 ± 0.0002
    0.150.150.170.230.42
    0.050.040.050.080.14
    0.020.020.010.020.04
MAbs 10F1 and 17C12
    37.0437.99 ± 0.00630.75 ± 0.001132.13 ± 0.014167.15 ± 0.01
    12.3512.15 ± 0.00210.90 ± 0.000713.45 ± 0.004647.25 ± 0.005
    4.124.03 ± 0.00053.91 ± 0.00024.68 ± 0.001121.46 ± 0.001
    1.371.61 ± 0.00021.50 ± 0.00011.74 ± 0.00017.27 ± 0.0002
    0.460.50 ± 0.00010.46 ± 0.00010.65 ± 0.00012.85 ± 0.0003
    0.150.10.10.191.03 ± 0.0002
    0.050.040.31 ± 0.0001
    0.020.05