TABLE 1.

Peptides used in this study

Protein and peptidePeptide sequenceaSpecies (Mka genotype)b
ESAT-6
    1 EAYQGVQQKWDATATE M. bovis
    1A --------------Q- M. kansasii (I-V)
    2 KWDATATELNNALQNL M. bovis
    2A ------Q--------- M. kansasii (I-IV)
    2B ------Q---S----- M. kansasii (V)
    3 LNNALQNLARTISEAG M. bovis
    3A --------S------- M. kansasii (I-IV)
    3B --S-----S------- M. kansasii (V)
CFP-10
    4 QEAGNFERISGDLKTQ M. bovis
    4A ------------M--- M. kansasii (IV)
    5 IDQVESTAGSLQGQWR M. bovis
    5A --------A---A--- M. kansasii (I-V)
    6 GSLQGQWRGAAGTAAQAA M. bovis
    6A A---A------------- M. kansasii (I-V)
    6B A---A------A------ M. kansasii (IV)
    7 AGTAAQAAVVRFQEAANK M. bovis
    7A --A--------------- M. kansasii (IV)
    8 VVRFQEAANKQKQELDEI M. bovis
    8A ------------A--E-- M. kansasii (I-V)
    9 QKQELDEISTNIRQAGVQYS M. bovis
    9A --A----E--------------- M. kansasii (I-V)
    10 NIRQAGVQYSRADEEQQQ M. bovis
    10A ----------K------- M. kansasii (I-V)
  • a -, amino acid residue in M. kansasii-derived peptide identical to the residue in the M. bovis sequence. Amino acids are represented by the one-letter code.

  • b M. kansasii genotype according to Arend et al. (4).