Patient | Diagnosis^{a} | ELISA result (value) for diagnosis^{b} | Immunoblot result^{c} | IgG ELISA result (value)^{b}^{,}^{d} | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|

IgM | IgG | Ct | N1 | N2 | N3 | C2 | C4 | C6 | VlsE | |||

NA6 | LA | P (6.90) | N | P | P (1.602) | N (0.146) | P (0.754) | N (0.107) | N (0.119) | N (0.097) | P (1.353) | P (0.275) |

NA7 | Clinical | P (1.93) | N | P | N (0.157) | N (0.119) | N (0.144) | N (0.102) | N (0.069) | N (0.105) | P (0.224) | N (0.081) |

NA8 | Clinical | P (4.41) | P | P | P (2.057) | P (0.803) | N (0.097) | N (0.160) | N (0.103) | N (0.106) | P (1.031) | P (0.319) |

NA9 | EM, LA | P (5.75) | N | P | N (0.091) | N (0.127) | N (0.0890) | N (0.087) | N (0.152) | N (0.144) | P (0.293) | N (0.090) |

NA10 | LA | P (7.48) | N | P | P (1.026) | N (0.207) | P (2.105) | N (0.168) | N (0.241) | N (0.154) | P (2.263) | P (1.450) |

NA11 | Clinical | P (3.51) | N | P | P (1.007) | N (0.321) | P (0.3) | N (0.099) | N (0.135) | N (0.214) | P (0.770) | P (0.440) |

NA12 | EM, clinical | P (4.33) | P | P | P (1.800) | N (0.079) | N (0.076) | N (0.117) | N (0.096) | N (0.093) | P (0.899) | P (0.639) |

NA13 | LA | P (8.45) | P | P | P (2.429) | P (0.433) | P (1.268) | P (1.495) | P (0.270) | P (0.271) | P (1.428) | P (1.605) |

NA14 | LA | P (5.49) | N | P | N (0.174) | N (0.305) | P (0.208) | N (0.175) | N (0.239) | P (0.258) | P (0.454) | P (0.192) |

NA15 | EM, clinical | P (5.98) | P | P | P (2.522) | N (0.331) | P (0.191) | P (2.086) | N (0.15) | N (0.13) | P (2.101) | P (2.136) |

NA16 | LA | P (8.18) | N | P | P (0.872) | N (0.123) | P (0.690) | N (0.061) | N (0.105) | N (0.113) | P (0.591) | P (0.207) |

NA17 | LA | P (3.77) | N | P | N (0.077) | N (0.152) | N (0.123) | N (0.082) | N (0.123) | N (0.111) | P (1.025) | P (0.220) |

NA20 | CSF C+ | P (5.82) | P | P | P (2.241) | P (0.602) | P (0.802) | P (0.275) | N (0.118) | N (0.116) | P (1.458) | P (0.853) |

NA22 | CSF C+ | P (1.24) | N | P | N (0.133) | N (0.191) | N (0.094) | N (0.066) | N (0.106) | N (0.135) | P (0.390) | N (0.090) |

NA18 | EM, C+ | P (2.58) | N | P | N (0.062) | N (0.233) | N (0.071) | N (0.064) | N (0.103) | N (0.088) | P (0.684) | N (0.123) |

NA19 | EM, C+ | P (1.89) | P | P | N (0.092) | N (0.213) | N (0.110) | N (0.087) | P (0.316) | N (0.139) | P (0.609) | N (0.112) |

NA21 | EM, C+ | P (3.87) | P | P | P (1.661) | E (0.420) | N (0.153) | P (0.225) | N (0.185) | N (0.171) | P (1.464) | P (0.195) |

NA23 | EM, C+ | P (4.77) | P | P | N (0.180) | P (0.486) | N (0.101) | N (0.083) | N (0.147) | N (0.176) | P (2.543) | P (1.045) |

NA24 | EM, C+ | P (3.91) | P | N | N (0.306) | N (0.256) | N (0.132) | N (0.127) | N (0.190) | N (0.158) | P (1.993) | P (0.160) |

NA25 | EM, C+ | P (3.49) | P | N | N (0.154) | N (0.152) | N (0.103) | N (0.082) | N (0.149) | N (0.126) | P (1.309) | N (0.106) |

NA26 | EM, C+ | P (2.80) | P | N | P (0.935) | P (1.073) | P (0.507) | P (0.483) | P (0.485) | P (0.435) | P (1.025) | N (0.150) |

NA27 | EM, C+ | P (1.45) | P | N | N (0.121) | P (0.460) | N (0.120) | N (0.096) | P (0.302) | N (0.123) | P (0.415) | N (0.099) |

NA28 | EM, C+ | P (2.27) | P | N | P (0.665) | N (0.281) | N (0.135) | N (0.109) | N (0.178) | N (0.168) | P (1.054) | N (0.137) |

NA29 | EM, C+ | N (0.53) | P | N | N (0.109) | N (0.131) | N (0.094) | N (0.073) | N (0.117) | N (0.109) | P (0.676) | N (0.084) |

NA30 | EM, C+ | P (1.75) | N | N | N (0.129) | N (0.186) | N (0.107) | N (0.075) | N (0.113) | N (0.104) | N (0.202) | N (0.106) |

NA31 | EM, C+ | E (0.99) | P | N | N (0.102) | N (0.106) | N (0.067) | N (0.065) | N (0.092) | N (0.093) | N (0.159) | N (0.065) |

NA32 | EM, C+ | E (0.95) | P | N | P (0.766) | N (0.289) | N (0.140) | N (0.125) | N (0.143) | N (0.142) | P (0.425) | N (0.117) |

NA33 | EM, C+ | P (1.30) | N | N | N (0.226) | P (0.547) | N (0.150) | N (0.144) | N (0.202) | N (0.187) | N (0.238) | N (0.126) |

NA34 | EM, C+ | P (1.58) | P | N | N (0.195) | N (0.133) | N (0.074) | N (0.082) | N (0.113) | N (0.143) | P (1.153) | N (0.110) |

NA35 | EM, C+ | P (1.59) | P | N | N (0.102) | N (0.091) | N (0.065) | N (0.088) | N (0.1) | N (0.089) | P (0.432) | N (0.089) |

NA36 | Rash, C+ | P (1.29) | N | N | N (0.183) | N (0.127) | N (0.088) | N (0.121) | N (0.131) | N (0.126) | N (0.180) | N (0.137) |

NA37 | EM, C+ | N (0.18) | N | N | N (0.114) | N (0.233) | N (0.072) | N (0.075) | N (0.124) | N (0.135) | N (0.159) | N (0.073) |

NA38 | EM, C+ | N (0.59) | N | N | N (0.407) | N (0.357) | N (0.153) | N (0.130) | N (0.173) | N (0.167) | P (0.637) | N (0.119) |

NA39 | EM, C+ | N (0.05) | N | N | N (0.157) | N (0.098) | N (0.077) | N (0.081) | N (0.115) | N (0.116) | N (0.173) | N (0.087) |

NA40 | EM, C+ | N (0.57) | N | N | N (0.212) | N (0.275) | N (0.077) | N (0.075) | N (0.102) | N (0.130) | P (0.356) | N (0.081) |

NA41 | EM, C+ | N (0.58) | N | N | N (0.167) | N (0.186) | N (0.111) | N (0.129) | N (0.194) | N (0.183) | N (0.318) | N (0.133) |

NA42 | EM, C+ | E (0.93) | N | N | N (0.096) | N (0.101) | N (0.109) | N (0.079) | N (0.116) | N (0.202) | N (0.270) | N (0.115) |

↵

*a*C+, culture positive; CSF, cerebrospinal fluid; LA, Lyme arthritis. Clinical diagnoses were made as described in Materials and Methods.↵

*b*P, positive; N, negative; E, equivocal (indeterminate). Bold values (positive) indicate samples with OD values greater than the mean plus 3 standard deviations of the negative control values.↵

*c*Lyme disease Marblot (MarDx).↵

*d*The values for control patients NA1 to NA5 were used to calculate the cutoff value (mean + 3 standard deviations). These were run in tandem on the ELISA plates with patient samples NA6 to NA31 or NA32 to NA42. The means + standard deviations (cutoff values) for these patients were as follows: for patients NA6 to NA31, 0.128 + 0.046 (0.265), 0.1842 + 0.079 (0.421), 0.096 + 0.024 (0.168), 0.116 + 0.025 (0.190), 0.150 + 0.039 (0.267), 0.126 + 0.032 (0.222), 0.115 + 0.036 (0.223), and 0.079 + 0.016 (0.127) for Ct, N1, N2, N3, C2, C4, C6, and VlsE, respectively; and for patients NA32 to NA42, 0.194 + 0.101 (0.497), 0.180 + 0.082 (0.425), 0.097 + 0.026 (0.173), 0.111 + 0.019 (0.168), 0.146 + 0.049 (0.292), 0.136 + 0.042 (0.260), 0.150 + 0.065 (0.344), and 0.085 + 0.025 (0.158) for Ct, N1, N2, N3, C2, C4, C6, and VlsE, respectively.