TABLE 1.

Recombinant BCG strains generated for expression of HIV-1 envelope

StrainDescriptiona
BCG-PBCG-P parent
222empPasEmpty vector pJH22 in BCG-P
120surPasCON6 gp120 for surface form in BCG-P
120intPasCON6 gp120 for intracellular form in BCG-P
120secPasCON6 gp120 for secreted form in BCG-P
140surPasCON6 gp140CF for surface form in BCG-P
140intPasCON6 gp140CF for intracellular form in BCG-P
140secPasCON6 gp140CF for secreted form in BCG-P
BCG-DBCG-D parent
222empDanEmpty vector pJH22 in BCG-D
120surDanCON6 gp120 for surface form in BCG-D
120intDanCON6 gp120 for intracellular form in BCG-D
120secDanCON6 gp120 for secreted form in BCG-D
140surDanCON6 gp140CF for surface form in BCG-D
140intDanCON6 gp140CF for intracellular form in BCG-D
140secDanCON6 gp140CF for secreted form in BCG-D
  • a All constructs have a kanamycin resistance gene for selection and were cloned into ApaI and HpaI restriction endonuclease sites in vectors. C, cleavage site deletion; F, fusion domain deletion. All gp140 constructs have the transmembrane and cytoplasmic domains deleted.