TABLE 2.

ELISA for P97c-specific antibody responses of BALB/c mice immunized with rAdP97ca

GroupMean OD405 ± SD (P)
SerumBAL
IgG on:IgA on day 60IgG on day 60IgA on day 60
Day 14Day 30Day 60
rAdGFP i.n.0.068 ± 0.0030.071 ± 0.00490.066 ± 0.00630.075 ± 0.0090.087 ± 0.0090.071 ± 0.002
rAdGFP i.m.0.065 ± 0.0020.071 ± 0.00630.058 ± 0.00140.058 ± 0.00140.081 ± 0.0150.067 ± 0.001
rAdP97c i.n.0.249 ± 0.003 (0.008)*0.36 ± 0.029 (0.026)*0.668 ± 0.046 (0.019)*0.106 ± 0.018 (0.054)0.781 ± 0.028 (0.012)*0.378 ± 0.037 (0.029)*
rAdP97c i.m.0.231 ± 0.005 (0.004)*0.487 ± 0.022 (0.0084)*1.138 ± 0.108 (0.0229)*0.106 ± 0.018 (0.054)0.587 ± 0.012 (0.01)*0.096 ± 0.022 (0.154)
  • a Groups of five mice were immunized with rAdP9c or rAdGFP (negative control) by the i.n. or i.m. route. Samples were collected at days 14, 30, and 60 and pooled by group. Sera were diluted 1:200 and BALs 1:50 and then analyzed for P97c-specific antibodies by ELISA in duplicate. *, significant difference (P < 0.05) compared with rAdGFP groups.