TABLE 3.

GMCs for ELISPOT responses with trypan blue viability, PHA ELISPOT response, and apoptosis AC limits applieda

AC measureDonor M3143Donor M5020
nGMCnGMC
VZVEPIVZVEPI
Trypan blue viability
    <89%752.010.36710.020.3
    ≥89%25276.0575.116178.2102.0
PHA response
    <1,000772.110.8433.814.5
    ≥1,00023394.8693.24089.955.6
Apoptosis
    >18%792.211.1689.720.1
    ≤18%21460.7756.315251.7117.0
  • a Results from 100 aliquots (donor M3143) or 83 aliquots (donor M5020) collected from a single donor bleed. Twenty aliquots optimally stored in an LN2 freezer (less than −130°C) and 34 to 40 aliquots for each donor were subjected to suboptimal storage by transfer to −20°C for 8 h or cycling between LN2 and −70°C storage (three cycles). n, number of aliquots.