TABLE 1.

Oligonucleotide primers for the reverse transcription and amplification of mRNA coding sequences for macaque SP-D, Acrp30, and GITRLa

PrimerSequenceSource and accession no.Location
macSP-D FWD5′-GCTGCTAGCATGCTGCTCTTCCTCCTCTCTGC-3′GenBank NM_003019172-194
macSP-D REV5′-GACTTCCTGGCCAAACTCCTGG-3′Trace MQAD1078F16.b1170-191
macSP-D Nested REV5′-GCTGTCTAGACCAAGCACTGCCCTACCCACC-3′Trace MQAD1078F16.b1193-214
macAcrp30 FWD5′-GCTGCTAGCATGTTGCTGGGAGCTGTTCTACTGC-3′GenBank AF4044071-24
macAcrp30 REV5′-TCCTAACTGTACTGAAAGCCTTTAATTGAC-3′GenBank NM_004797891-863
macAcrp30 Nested REV5′-GCTGTCTAGAGACTTTGGGGCTGTTTGGCCTG-3′GenBank NM_004797865-844
macGITRL FWD5′-CAGCTCTTATTTCTCCAAAAATGTGTTTGAGC-3′Trace RHJHK27TF650-681
macGITRL REV5′-CCACCCACTGGCACCTCTG-3′GenBank BV209630456-438
macGITRL Nested FWD5′-GCTGCTAGCATGTGTTTGAGCCACTTGGAAAATATGCC-3′Trace RHJHK27TF670-698
macGITRL Nested REV5′-GCTGTCTAGACCTCTGCATGTGCTGAAGGGAAT-3′GenBank BV209630444-421
  • a Where possible, primer sequence was obtained from annotated macaque mRNA sequence data. Alternately, primers were designed from unannotated sequence data available from the NCBI macaque trace sequence archive (Trace) or annotated sequence for the human orthologs.