TABLE 1.

Raw viability (V) and yield (Y) data (%) from the seven single-donor QAP roundsa

LabDonorQAP roundImprovement (slope)b,c
1234567
VYVYVYVYVYVYVYVY
HIV+937883142951059411189418965−0.13−5.88
HIV9410197689076977995108965798490.54−5.39
Local9880955793100
B‡HIV+72326522508734151693232.94−1.24
HIV61309240542585534523826298452.933.11
Local7331
HIV+9254865873688885963190670.860.30
HIV83417854945675998440905491591.381.36
Local846688448573
HIV+96669262935989102771199577−0.964.49
HIV94711007210077968173122881419751−1.500.68
Local92124971449861
F‡HIV+7662502776286746834965670.563.21
HIV75237725632467958868928690633.5710.21
Local9711398939790
J‡HIV+00692391837698906914.3115.37
HIV138147759664857711676618.343.20
Local846994749661
K‡HIV+0097113855615.427.39
HIV0086185242918612.007.40
Local6458351397108
LHIV+81508472§§§§
HIV9542701166745−14.01.50
Local
MHIV+5521292218§§−1.14
HIV140.6144019−7.002.22
Local9612765269549
N‡HIV+70187644073§§−5.36
HIV761856626971458.215.29
Local8498494
O‡HIV+743277587535882586752.225.33
HIV88393622816070328571841042.9410.25
Local93689272
PHIV+9671§§§§
HIV91789863§§§§
Local83429481
QHIV+9117§§§§
HIV9513§§§§
Local83428814
QAP round summary (mean ± SD)HIV+74 ± 1154 ± 1964 ± 3247 ± 3366 ± 3040 ± 3381 ± 1368 ± 3272 ± 2151 ± 3388 ± 957 ± 23
HIV83 ± 1351 ± 2970 ± 3347 ± 2170 ± 3246 ± 2776 ± 2371 ± 3467 ± 2561 ± 3872 ± 3160 ± 3492 ± 758 ± 29
Local85 ± 1060 ± 2984 ± 2162 ± 3094 ± 472 ± 28
  • a For statistical analysis, the maximum yield was defined as 100%. Improvements in PBMC viability and yield from each laboratory (single-donor PBMC only) were calculated by linear regression analysis (slope), and overall improvement was determined from the slope data by a t test. Laboratories A, C, and E (§) retained a sufficient level of performance throughout each QAP round (P > 0.1). Most remaining laboratories showed substantial improvements (‡) in viability and yield results.

  • b §§, insufficient data for slope analysis.

  • c P values for a slope t test for all labs were 0.100 and 0.020 for viability and yield, respectively, and those for a t test for improved labs were 0.002 and 0.001 for viability and yield, respectively.