TABLE 1.

Frequencies of X4 HIV-1 (NL4-3) and R5 HIV-1 (JR-CSF) expression in thymocyte subsets in SCID-hu micea

VirusWkFrequency of virus expression in indicated thymocyte subset
Subset I-PSubset ISubset IISubset IIISubset IV
X4 HIV-1 (NL4-3)2.50.080.040.030.020.01
2.50.60.30.130.160.2
428.818.25.455.5
427.517.94.35.36.5
424.818.85.37.28.7
52618.824ND1.5
52310.74.5ND4.5
R5 HIV-1 (JR-CSF)3.50.50.30.43.12
3.50.60.30.42.45.1
40.50.30.62.72.7
40.70.61.13.13.6
40.50.61.02.72.3
40.70.20.42.62.4
41.20.20.32.22.1
42.30.3121.4
71.00.51.1ND1.1
72.10.92.3ND1.6
73.21.11.4ND1.3
70.40.20.6ND1.8
  • a Thymocytes were isolated from HIV-1-infected implants from 2.5 to 7 weeks postinfection. The cells were surface stained with antibodies to CD3, CD71, CD69, CD27, and CD45RA combined with intracellular staining for the GagHIV-1 protein (KC57-FITC). Each specific subset was gated electronically, and the frequencies of GagHIV-1 (KC57+)-expressing cells in the subsets were determined. ND, not determined.