TABLE 1.

Plasmid constructs generated for expression of HIV-1 CON6-gp120 and -gp140 in M. smegmatis

VectorDesigned for:MarkerInsert(s)Cloning site
pMV261Intracellular expressionKanamycinCON6-gp120 and CON6-gp140CFBamHI/HpaI
pMV361Chromosomal integrated vectorKanamycinCON6-gp120 and CON6-gp140CFEcoRI/HpaI
pJH152Surface expressionKanamycinCON6-gp120 and CON6-gp140CFApaI/HpaI
pJH153Intracellular expressionKanamycinCON6-gp120 and CON6-gp140CFApaI/HpaI
pJH154Secreted expressionKanamycinCON6-gp120 and CON6-gp140CFApaI/HpaI
pYUB2051Intracellular expressionHygromycinCON6-gp120 and CON6-gp140CFApaI/HpaI
pYUB2052Surface expressionHygromycinCON6-gp120 and CON6-gp140CFApaI/HpaI
pYUB2053Secreted expressionHygromycinCON6-gp120 and CON6-gp140CFApaI/HpaI
pJH222Surface expressionKanamycinCON6-gp140CFNdeI/SpeI