TABLE 1.

Flow cytometric analysis of responses to B. abortus strain 19 after initial and booster vaccinationa

Time postvaccination and animal groupMean no. of proliferating cells/10,000 PBMC ± SEMb
TotalCD4+CD8+γδTCR+IgM+
14 wk after initial vaccination
    Controls893 ± 407126 ± 4625 ± 1122 ± 15881 ± 174
    RB51 vaccinates1,833 ± 579*449 ± 35119 ± 15335 ± 3111,927 ± 432*
    S19 vaccinates1,262 ± 46764 ± 4264 ± 6459 ± 591,942 ± 607*
22 wk after initial vaccination
    Controls367 ± 367353 ± 32576 ± 4238 ± 27273 ± 184
    RB51 vaccinates2,161 ± 1,187*1,354 ± 384*381 ± 101*89 ± 891,277 ± 333*
    S19 vaccinates2,063 ± 405*2,282 ± 245*529 ± 25311 ± 931,873 ± 212*
12 wk after booster vaccination
    Controls0 ± 0404 ± 32288 ± 3428 ± 42141 ± 30
    RB51 vaccinates0 ± 0808 ± 488232 ± 1210 ± 0467 ± 196
    S19 vaccinates1,000 ± 541691 ± 303306 ± 13424 ± 41637 ± 266
  • a PKH-67-labeled PBMC from elk vaccinated s.c. with saline (control), SRB51, or S19 (n = seven animals/treatment) and booster vaccinated 65 weeks later were incubated with 108 CFU of gamma-irradiated B. abortus strain 19 at 37°C and 5% CO2 for 7 days, labeled with monoclonal antibodies, and analyzed in a flow cytometer.

  • b Means within a sampling time with an asterisk are significantly different (P < 0.05) from means of controls labeled with the same monoclonal antibody.