Apparent disease prevalence in M. avium subsp. paratuberculosis-infected herds by test result

Test methodNo. of cows tested% Test positive% Heavy fecal sheddersa
At least 1 culture test positive1,55325.45.3
All 3 culture tests positive1,7775.0NAb
Culture using centrifugation1,80818.45.6
Culture using sedimentation1,80710.13.9
Culture using BACTEC1,4819.64.5
Fecal PCR1,8087.6NA
Serum ELISA A1,70610.5NA
Serum ELISA B1,7047.9NA
Milk ELISA1,5767.9NA
  • a Heavy fecal shedder defined as 3+ to 4+ (see Materials and Methods).

  • b NA, not applicable.