TABLE 1.

Bacterial growth in the lungs and spleens of vaccinated and control micea

Immunization groupLungsSpleens
Mean log10 CFU viable bacteria ± SEbDifference in CFUMean log10 viable bacteria ± SEDifference in CFU
Unvaccinated     control5.146 ± 0.065.459 ± 0.08
wt PR8 virus5.279 ± 0.10−0.1335.491 ± 0.01−0.077
Flu/ESAT     (H1 + H3)3.778 ± 0.08*1.3694.859 ± 0.12*0.60
BCG3.873 ± 0.12*1.2734.939 ± 0.12*0.520
  • a Protective efficacy in C57BL/6 mice immunized with Flu/ESAT-6 (H1 + H3), wt PR8 virus, or BCG was determined 40 days after i.v. infection with virulent M. bovis.

  • b Asterisks show the statistical significance (P < 0.05) compared to nonvaccinated controls, based on the unpaired, two-tailed Student t test.