TABLE 1.

Comparison of IgG and IgM antibody responses to rMS-HBHA and Ag85B proteins in TB patients and healthy controls

Patient group (no. of subjects)ELISA result fora:
rMS-HBHAAntigen 85B
IgGIgMIgGIgM
Mean ODb ± SDNo. (%) of positive serum samplescMean OD ± SDNo. (%) of positive serum samplesMean OD ± SDNo. (%) of positive serum samplesMean OD ± SDNo. (%) of positive serum samples
Chronic TB (21)0.23 ± 0.15†d8 (38)0.26 ± 0.39†7 (33)0.87 ± 0.42†20 (95)0.05 ± 0.04†2 (10)
Early TB (33)0.15 ± 0.07‡5 (15)0.09 ± 0.07‡4 (12)0.35 ± 0.26‡16 (49)0.04 ± 0.03‡1 (3)
Healthy controls (33)0.08 ± 0.05§1 (3)0.07 ± 0.05‡0 (0)0.11 ± 0.06§0 (0)0.03 ± 0.02‡1 (3)
  • a Sera were collected both from TB patients who had been treated for >1 year (chronic TB) and <1 month (early TB) and from healthy controls and were diluted 1:80.

  • b Values are expressed as means of OD450 ± SD.

  • c The cutoff value for IgG and IgM antibodies to each antigen was calculated as the mean OD obtained with sera from 33 healthy controls plus 3 SD.

  • d Duncan's test for multiple comparisons of means was used to determine whether there were significant differences between the results for the three groups. The different symbols (†, ‡, §) indicate significant differences (P < 0.05) between the means of antibody titers for the three groups.