TABLE 3.

Comparison of the RRV-specific epitope-blocking ELISA and standard diagnostic techniques for detection of antibodies to RRV in human seraa

Serum no.% MAb inhibitionHI titerImmunoglobulin MbSerum no.% MAb inhibitionHI titerImmunoglobulin Mb
5001237Z0<105020602Y99640+
5001709Y2640+5020667R96160+
5001710Z5<105020669T7780+
5002634Z25160+5020680T3080+
5003475S8680+5020958X88160+
5005725Z7540+5023355W9080+
5005726N62160+5023917X95160+
50057327340+5022402N4240+
500573696320+5024567Y84640+
5005979N42160+5025726Q0<10− (BFV+)
5002137X4105025727R0<10− (BFV+)
5005721U91160+5025859R3880+
5003914R5740+5025866P87320+
500373473160+5025872T93160+
5006620R78160+5026087N3740+
5007112N040+5027036R010NT
5007587Q20<105027498R8840− (RRV+)
501045484>640+5027993R89640+
501081398160+5028013P9080+
501084995160+5028040U81640NT
5012156U6580+5028481W1080+
5012919Z9180+5029421P8880+
5012941Z8180+5029446S59320+
5013411W87640+5029423R95320NT
5013914S90160+5030507Y92640+
501341876160+5030539X9180+
5013430S93160+5030985R89320+
5013438P88160+5031004N3480+
5013919Y82160+5031017Q85160+
5016123Q9080+5031453Y96320+
5016283X92160+5032419W9640− (RRV+)
5018282T94320+5032916Y95320− (RRV+)
5018285X9080+5033430U9540− (RRV+)
5019288Z8880+5033659S9180− (RRV+)
5018296X95640+5034376U9140− (RRV+)
5018292S0<10− (BFV+)5034746Q96>640− (RRV+)
5019113N3640+5034773W93>640− (RRV+)
5020529X90160+5035191W5840− (RRV+)
5020658T4080+
  • a Boldface indicates discrepancies between ELISA, HI, and immunoglobulin M results.

  • b (RRV+), negative for immunoglobulin M but positive for immunoglobulin G; (BFV+), positive for antibodies to BFV; NT, not tested.