TABLE 4.

Comparison of the epitope-blocking ELISA and standard diagnostic techniques for the detection of RRV antibodies in paired serum samplesa

Serum no.Serum pair no.% InhibitionHI titerImmunoglobulin M
5001237Z10<10
5003914R5740+
5001710Z25<10
500373473160+
5002137X3410
5006620R78160+
5007112N4040+
501341876160+
5007587Q520<10
5013919Y82160+
5019113N63640+
5023917X95160+
5022402N74240+
5028040U81640NTb
5026087N83740+
5030985R89320+
5027036R9010NT
5029446S59320+
  • a Nine paired samples are listed sequentially as acute- and convalescent-phase samples. Boldface indicates discrepancies between blocking ELISA, HI, and immunoglobulin M results. The PathCentre Laboratory, which supplied these paired serum samples, requests that doctors obtain a convalescent-phase serum 14 days after the acute-phase specimen was taken. Although this is ideal, it is not always possible, and the convalescent-phase sera used in this study were taken between 5 and 18 days after the acute-phase sample.

  • b NT, not tested.