TABLE 1.

Neutralization titer and percent inhibition of MAb binding in the epitope-blocking ELISA produced by rabbit antisera to reference RRV strains and other alphaviruses

Rabbit antiserum (location and yr of strain isolation)Neutralization titerb% Inhibitiona of MAb:
3B2cG8B10
RRV strains
    T48 (North Queensland, 1959)160 56 4667
    NB5092 (Eastern New South Wales, 1969)160 56.5 6264
    Ch19575 (western Queensland, 1976)640 75.5 540
    K1503 (East Kimberly, WA, 1984)320 65 170
    WK20 (West Kimberley, WA, 1977)320 67 180
    SW876 (southwest WA, 1987)160 50 8261
    SW2191 (southwest WA, 1988)80 76 420
Other alphaviruses
    Getah virus480 6.5 110
    Sindbis virus40 0 480
    BFV640 0 590
    Semliki Forest virus640 4 700
    Chikungunya virus40 0 700
  • a The percent inhibition of MAb binding was calculated as 100 − [OD (test)/OD (negative control) × 100].

  • b Against homologous virus.

  • c Boldface indicates that the RRV-specific MAb 3B2 produced the best results.