TABLE 1.

Kinetics of the isotype response to MPB83 as detected by MAPIA and corresponding VetTB STAT-PAK test results

AnimalWk after inoculationaMAPIA resultbVetTB STAT-PAK test resultb,c
IgMIgG
1930
3++
6+++++++++
12+++++++
25++++++
48++++
78++++++
1980
3++
6+++++++
12++++++++
25++++++
48++++
78++++++
  • a Cattle received 106 CFU of M. bovis strain AF2122/97 via intranasal administration.

  • b Responses indicate the relative intensities of bands, with −, +, ++, and +++ indicating no, weak, moderate, and intense reactivities, respectively.

  • c The test utilizes a unique cocktail of several recombinant antigens, including MPB83, ESAT-6, and CFP-10, optimized by evaluation of antigen recognition patterns using MAPIA.