TABLE 1.

Reactivities of monoclonal antibodies with E. bieneusi spores

MAbResult with:b
Frozen MAbaSpore fixation methodDeglycosylation (periodate)Deglycosylation (enzymatic)c
MethanolParaformaldehydeAcetone
1D4+++++++++++++++++++
3E9++++++++++++++++++++
2B2+++++++++++++++++++
2G4+++++++++++++++++
2E9+++++++++++++++++++
4B2+++++++++++++++++
4C9++++++++++++++++++++
4C3+++++++++++++++++++
  • a Reactivities of the MAbs frozen at −70°C were tested after every month for 3 months. Reactivity intensities remained the same during that period.

  • b +, faint fluorescence intensity; ++, weak fluorescence intensity; +++, strong fluorescence intensity; ++++, very strong fluorescent intensity.

  • c Enzymatic deglycosylation removed N-linked, simple O-linked, and complex O-linked carbohydrates but did not alter reactivity of any MAb even after 5 days of deglycosylation.