TABLE 3.

Comparative sensitivities of IFA (using three different MAbs) and PCR in serially diluted feces containing E. bieneusi spores

Serial dilutionResultSpore count/10 microscopic fieldsaEstimated no. of spores/g of feces
Primary PCRNested PCRIgG IFA (IB7; IgG2a)IgG IFA (12G8; IgG2b)IgM IFA (2G4; IgM)
Undiluted++5974801,5373.8 × 107
1:2++4534151,2801.9 × 107
1:4++2812248459.5 × 106
1:8++1811244424.8 × 106
1:16++1381073282.4 × 106
1:32++87581181.2 × 106
1:64++3029386.0 × 105
1:128++2619263.0 × 105
1:256++159201.5 × 105
1:512+0007.5 × 104
1:1,0240003.8 × 104
1:2,0480001.9 × 104
Control0000
  • a At a magnification of ×400.