TABLE 1.

ELISA IgG concentrations and corresponding avidity measurements for a panel of 16 sera after elution or binding interference with 0.15 M NaSCN

SerumaElution with 0.15 M NaSCNBinding interference with 0.15 M NaSCN
IgG concn (μg/ml) at:% Reduction in IgG concnbIgG concn (μg/ml) at:% Reduction in IgG concnb
0 M NaSCN0.15 M NaSCN0 M NaSCN0.15 M NaSCN
113.997.8743.712.847.5341.4
216.89 10.89 35.514.92 13.24 11.3
32.050.7762.42.010.5771.6
43.481.6652.32.841.4449.3
51.040.5349.00.780.4344.9
66.21 4.59 26.15.49 0.84 84.7
74.662.3649.44.382.0353.7
8569.98132.9976.7555.8127.577.1
9177.92 98.31 44.7169.74175.89−3.6
109.795.5843.08.965.6437.1
119.265.738.47.474.2842.7
1232.1312.361.729.6910.3265.2
1310.264.1559.68.183.6954.9
147.781.9574.97.931.6679.1
1540.6121.0748.137.1815.2459.0
1612.44 8.9 28.59.15 13.35 −45.9
PSAB-9070.735.749.566.030.953.1
  • a Sera 1 to 7 were prevaccination and sera 9 to 16 were postvaccination sera. PSAB-90 results represent the mean of four independent assays. Values for sera with high CVs (>20%) are boldfaced.

  • b Percent reduction in IgG antibody concentration (μg/ml) in the presence of the chaotrope NaSCN (0.15 M).