TABLE 3.

Association of the tuberculin-boosted antibody responses to M. bovis antigens with pathology/bacteriology findings in experimentally infected reindeer

AnimalResults for antibodies in MAPIAPathology and bacteriology results
Band intensityaNo. of bandsMAPIA scorebGross lesionsCulture from tissuesNo. of positive tissuescPathology scored
121+++47PositivePositive35
126+++69PositivePositive57
128e+++47PositivePositive46
120++46NegativeNegative11
131++46NegativeNegative11
133++46NegativeNegative11
134++35NegativeNegative33
135++35NegativeNegative00
125+/−22.5NegativeNegative22
127+45PositivePositive13
136+34NegativeNegative00
  • a Magnitude of antibody response to most seroreactive antigen(s) detected by MAPIA 2 to 4 weeks after skin testing performed 3 months postinfection was scored visually as strong (+++), moderate (++), weak (+), or very weak (+/−).

  • b MAPIA score is the band intensity (e.g., +++ represents 3 and +/− represents 0.5) plus number of bands.

  • c At necropsy (13 months postinfection), 15 tissue specimens, including tonsil, lung, spleen, liver, kidney, and 10 different lymph nodes, were examined for gross lesions consistent with tuberculosis and histopathology (caseonecrotic granuloma with acid-fast bacilli); the tissues were also used to isolate M. bovis by bacteriologic culture; disease status was confirmed for all 11 infected reindeer by positive histopathology findings in one or more tissue specimens (not shown). Numbers of tissues with positive gross pathology and/or bacteriology results are shown.

  • d The pathology score is the value for gross lesions plus the value for culture from tissues plus the number of positive tissues. Positive and negative findings were assigned values of 1 and 0, respectively. Pearson's correlation coefficient was 0.68 (P = 0.02) for the comparison of MAPIA scores to pathology scores.

  • e Euthanized 7 months postinfection due to complications unrelated to M. bovis inoculation.