TABLE 3.

Antibody detection in known singly positive sera by multiplex microbead assaya

Serum sampleDilutionMFI ratio of antigen beads to BSA beads for antigen-coated microbead set:
Vaccinia virusNCDVReo-3MPMHVSendai virusGD7MPVCMVMMV
Background0.280.230.310.130.260.580.240.170.250.50
Ectro1:2 × 104b167.00.030.000.030.000.050.000.000.000.00
EDIM1:2500.1963.00.290.090.080.430.030.110.110.42
Reo-31:2500.140.16189.00.160.160.270.000.010.150.34
MP1:2500.130.130.2919.00.230.100.000.000.340.73
MHV1:2500.080.040.300.1112.00.100.000.100.270.33
Sendai virus1:2500.170.240.350.140.18153.00.000.000.060.44
GD71:2500.070.150.260.150.110.3712.00.020.090.35
MPV1:2500.090.110.250.080.080.170.0011.00.010.31
CMVAD0.050.050.180.080.640.080.000.0427.00.06
MMV1:2500.220.200.620.140.300.170.350.200.215.00
  • a Results are averages of two independent experiments. Boldface indicates the most specific results. Abbreviations: MP, M. pulmonis; Ectro, ectromelia virus; AD, as directed by the manufacturer (Charles River, MA).

  • b Higher dilution needed due to high antibody titer.