TABLE 1.

Production of standard sera in BALB/cj mice

PathogennInoculation route-doseaSeroconversionb (% positive)
PrimarySecondaryFinalELISAMultiplex
Reo-324i.n.−1.0 TCID50i.n.−1.0 TCID50None100100
Sendal virus4i.n.−1 μg/10 μlNoneNone100100
18i.n.−1 μg/10 μli.n.−1 μg/10 μlNone100100
GD73i.n.−2 × 106 PFU/10 μlNoneNone100100
4i.n.−6 × 106 PFU/10 μlNoneNone100100
4i.n.−3 × 106 PFU/10 μli.n.−6 × 106 PFU/10 μlNone100100
4i.n.−6 × 106 PFU/10 μli.n.−6 × 106 PFU/10 μlNone100100
4i.n.−2 × 106 PFU/10 μli.n.−2 × 106 PFU/10 μli.n.−6 × 106 PFU/10 μl100100
MHV10d.b.NoneNone6050
12d.b.d.b.None100100
45 d.b. inoculations at 2-wk intervals100100
156 d.b. inoculations at 2-wk intervals100100
EDIM11g.l.−1 μg/100 μli.p.−1 μg/100 μli.p.−1 μg/100 μl100100
4g.l.−3 μg/100 μlg.l.−3 μg/100 μli.p.−3 μg/100 μl100100
4g.l.−6 μg/100 μlg.l.−6 μg/100 μli.p.−6 μg/100 μl100100
3g.l.−9 μg/100 μlg.l.−9 μg/100 μli.p.−9 μg/100 μl100100
M. pulmonis4i.n.−40 μg/10 μli.n.−40 μg/10 μli.p.−40 μg/10 μl100100
4i.n.−77 μg/10 μli.n.−77 μg/10 μli.p.−77 μg/10 μl100100
4i.n.−150 μg/10 μli.n.−150 μg/10 μli.p.−150 μg/10 μl100100
12i.p.−150 μg/10 μli.p.−150 μg/10 μlNone92100
Vaccinia virus24i.p.−1 μg/100 μli.p.−1 μg/100 μli.p.−1 μg/100 μl9191
MMV12i.n.−1.0 TCID50i.n.−2.0 TCID50i.p.−2.0 TCID504141
12i.n.−1.0 TCID50i.p.−2.0 TCID50i.p.−2.0 TCID507575
24i.p.−3.0 TCID50i.p.−3.0 TCID50i.p.−3.0 TCID50100100
  • a i.n., intranasal; d.b., dirty bedding; g.l., gastric lavage; TCID50, 50% tissue culture infectious dose.

  • b For simplicity, only the final seroconversion results are presented.