TABLE 3.

Intra-assay accuracy of NK-1R real-time RT-PCR for the specimensa

ReplicateNK-1R mRNA (copy no.)
D1D2D3Cell line
PBLMDMPBLMDMPBLMDMIM9NT-2N
13,0901,8659,3621,84620,1363,241171,4721,717,887
22,4211,6408,4481,52216,6073,139106,6081,435,145
33,0121,3619,8561,85818,7633,478110,0871,363,270
42,8431,4249,6061,41916,6073,733120,4441,425,958
Mean (± SD)2,842 (± 299)1,573 (± 229)9,318 (± 614)1,661 (± 224)18,022 (± 1,730)3,398 (± 265)127,153 (± 3,0125)1,485,565 (± 158,40)
CVb (%)10.5214.556.5813.489.607.8023.691.10
  • a D, donor; healthy peripheral blood. Abbreviations: PBL, peripheral blood lymphocytes; MDM, monocyte-derived macrophages; IM9, human B lymphoblasts; NT-2N, human neuronal cells derived from Ntera2/cl.D1 (NT2) cells.

  • b CV, coefficient of variation.