TABLE 2.

Inter-assaya accuracy of NK-1R real-time RT-PCR

ReplicateThreshold cycle of input copies
10,000,0001,000,000100,00010,0001,000100
116.7819.8323.2726.7430.4934.18
216.9919.4923.0927.0931.1934.43
316.8919.7723.0926.9730.9634.34
417.2220.0623.2427.6630.434.92
Mean (± SEM)16.97 (± 0.09)19.79 (± 0.12)23.17 (± 0.05)27.12 (± 0.20)30.76 (± 0.19)34.47 (± 0.16)
  • a The data are generated from four separate assays performed on different days.