TABLE 4.

Multiplex microbead assay on mixed, singly positive mouse sera from a

Serum sampleDilutionbMFI ratio of antigen beads to BSA beads for antigen-coated microbead set:
Vaccinia virusNCDVReo-3MPMHVSendai virusGD7MPVCMVMMV
Background0.280.230.310.130.260.580.240.170.250.50
All sera1:25041.825.397.610.97.4070.35.654.4513.13.48
Ectro (−)1:2500.3323.193.012.56.0060.85.604.3012.83.30
EDIM (−)1:25042.10.3993.912.06.7065.235.604.0013.23.10
Reo-3 (−)1:25043.123.920.2912.87.3469.66.064.6213.43.37
MP (−)1:25055.332.71350.249.7089.08.116.0017.13.50
MHV (−)1:25041.023.796.812.10.8265.95.674.2112.63.52
Sendai virus (−)1:25045.824.210012.77.140.656.204.6013.03.10
GD7 (−)1:25043.624.196.012.06.8066.40.044.3012.83.27
MPV (−)1:25043.522.896.511.66.8162.45.640.0012.73.20
CMV (−)1:25045.623.893.710.86.1168.05.504.220.482.90
MMV (−)1:25041.022.210011.87.0166.77.704.1312.41.56
  • a Results are averages of two independent experiments. MP, M. pulmonis; Ectro, ectromelia virus. Boldface indicates the least specific results.

  • b Final dilution of each serum sample in the mixture.