TABLE 5.

Multiplex assay for serodetection in field samples of micea

Serum sampleDilutionMFI ratio of antigen beads to BSA beads for antigen-coated microbead set:
Vaccinia virusNCDVReo-3MPMHVSendai virusGD7MPVCMVMMV
Background0.130.330.510.260.630.870.310.080.850.71
40291:2500.1334.5b,c0.68e0.1423.3b,c0.0913.8b,c0.000.550.31
40301:2500.0514.3b,c0.150.0819.6b,c0.0311.1b,c0.000.530.16
40311:2500.3413.0b,c0.280.2025.5b,c0.4311.7b,c31.0b,c0.810.52
40321:2500.1434.7b,c0.000.1524.5b,c0.189.11b,c48.4b,c0.453.43b,c
40331:2500.169.52b,c0.55e0.3021.7b,c0.120.050.000.382.30b
40341:2500.147.60b,c0.150.1629.2b,c0.109.60b,c151b,c0.751.67b,c
40351:2500.093.74b,c2.13b,d0.1714.1b,c0.092.72b,c46.8b,c0.260.44
  • a Results are averages of two independent experiments. MP, M. pulmonis. Serum samples are from individual mice.

  • b Positive by the multiplex microbead assay.

  • c Confirmed as positive by ELISA.

  • d Negative by ELISA but confirmed positive by IFA.

  • e Negative by ELISA and indeterminate by multiplex assay and IFA.