TABLE 2.

Endpoint antibody titration by the rapid c-ELISA and VNT of sera from animals experimentally immunized with PPRV or RPV

Serum sampleVNT titerac-ELISA titerb
PPRV hyperimmune serum
    αPPRV-I>1:5121:512
    αPPRV-II>1:5121:512
    αPPRV-III>1:512>1:512
    αPPRV-IV>1:512>1:512
RPV hyperimmune serum
    αRPV-I>1:51216
    αRPV-II>1:5128
    αRPV-RBOK1:1,2802
RPV-vaccinated serumc
    K90611:256<2
    K90621:180<2
    Rb0011:22<2
    Rb0021:16<2
    Rb0031:45<2
    Rb0041:64<2
    Rb0051:32<2
    Rb0061:32<2
    Rb0071:22<2
    Rb0081:32<2
    Rb0091:16<2
    Rb0101:16<2
  • a VNT was with homologous viruses PPRV Nig75/1 for PPRV and RPV LATC for RPV.

  • b The titer represents the highest serum dilution showing PI values of ≥50.

  • c Two cattle (animals K9061 and K9062) were vaccinated twice, with a 3-week interval between inoculations, and the other animals (animals Rb001 to Rb010) received one inoculation each. All cattle were bled 3 weeks after the last vaccination. RPV strain LATC was used.