TABLE 1.

Intra-assaya accuracy of NK-1R real-time RT-PCR

ReplicateThreshold cycle of input copies
10,000,0001,000,000100,00010,0001,000100
116.919.723.426.830.233.9
216.920.123.326.630.433.5
316.819.623.127.030.434.4
416.420.023.227.031.035
Mean (± SD)16.7 (± 0.2)19.8 (± 0.2)23.3 (± 0.1)26.7 (± 0.2)30.5 (± 0.4)34.2 (± 0.7)
CVb (%)1.381.110.560.751.181.9
  • a Four replicate samples from each dilution were amplified in the same plate.

  • b CV, coefficient of variation.