Subject | Epitope | Relationship between relative affinity and ID_{50}^{a} | No. of sera examined | r^{2} |
---|---|---|---|---|

R | TB71 | Log-linear^{b} | 9 | 0.978 |

W | TB71 | Exponential^{c} | 8 | 0.970 |

TB72 | Log-linear | 8 | 0.709 | |

M | TB71 | Log-linear^{c} | 7 | 0.891 |

TB72 | Log-linear | 7 | 0.934 | |

F | TB23 | Log-linear^{d} | 7 | 0.885 |

Wi | TB23 | Exponential^{d} | 7 | 0.865 |

TB71 | Log-linear | 7 | 0.747 | |

S | TB71 | Log-linear | 7 | 0.515 |

A | TB68 | Log-linear | 7 | 0.676 |

V^{e} | TB23 | Log-linear | 6 | 0.893 |

C | TB78 | Log-linear | 6 | 0.603 |

TB71 | Log-linear | 5 | 0.669 | |

H | L4^{f} | Log-linear | 5 | 0.845 |

↵

*a*A log-linear relationship indicates no change in affinity of the antibody, whereas an exponential relationship indicates affinity maturation during treatment.↵

*b*As shown in Fig. 5B.↵

*c*As shown in Fig. 5C.↵

*d*These two subjects had antibody levels within the range defined by healthy controls for three or four initial samples and then positive titers for later samples.↵

*e*Same individual as V71 in Fig. 5C.↵

*f*Monoclonal antibody L4 binds to the same epitope as ML34 but is an IgG antibody, and therefore analysis of the serum could be carried out at the same time as for the other IgG monoclonal antibodies.