TABLE 1.

Results of Brucella IgM and IgG flow assays for different groups of sera and comparison with SAT, DTT-SAT, and Coombs

Serum group (no. of samples)No. (%) of sera testing positive by indicated assay
SAT, ≥1:320SAT, ≥1:160SAT-DTT, ≥1:160SAT-DTT, ≥1:80SAT-DTT, ≥4-fold reduction in titerCoombs, ≥1:320IgM flow, ≥1+IgG flow, ≥1+IgM + IgG flow, ≥1+f
Initial samples from patients with clinical suspicion of brucellosis
    Albacete (54)31 (57)39 (72)24 (44)31 (57)25 (46)53 (98)28 (52)33 (61)41 (76)
    Malaga (81)43a (57)57a (76)45b (62)53b (73)20 (27)b68 (94)d29 (36)54 (67)67 (87)
    Pamplona (70)52c (78)58c (87)53c (79)49c (79)30 (45)c66 (99)c47 (67)50 (71)65 (93)
    Total (205)127 (65)154 (79)122 (63)133 (69)75 (38)187 (97)104 (51)137 (67)173 (84)
Rev-1 contacts (10)0 (0)0 (0)NTeNT0 (0)6 (60)0 (0)4 (40)4 (40)
Patients with fever from an area of nonendemicity for brucellosis (79)NTNTNTNTNTNT1 (1)1 (1)1 (1)
Cases with illnesses other than brucellosis (197)NTNTNTNTNTNT2 (1)1 (1)2 (1)
  • a Six sera were not tested.

  • b Eight sera were not tested.

  • c Three sera were not tested.

  • d Nine sera were not tested.

  • e NT, not tested.

  • f Positive (≥1+) test result in at least one of the two flow assays.